Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: List of CD4 enumeration platforms and antibody reagents tested with commercial stabilized whole blood products.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Stability of Multi-Check , StatusFlow and CD4 Count stabilized whole blood products (SWBPs) under different conditions on various platforms.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: PLoS ONE

Article Title: Compatibility of Stabilized Whole Blood Products with CD4 Technologies and Their Suitability for Quality Assessment Programs

doi: 10.1371/journal.pone.0103391

Figure Lengend Snippet: Multi-Check , StatusFlow and CD4 Count (low CD4 level) were prepared using the CD3/CD4 reagent kit on product stored at 4°C (D0) and products stored for 1 day at 37°C (D1). Analysis required first setting cursors around the CD3 cells population FSC×CD3 PECy5 dot plot and then isolating the CD4 positive cells cluster on CD4PE×CD3PECy5.

Article Snippet: Stability was evaluated using the following reagent kits: the MultiTest Reagent on the FacsCalibur, the FACSCount Reagent kit and FACSCount CD4 Reagent kit on the FACSCount (BD Biosciences, San Jose, CA), the Guava Express CD3/CD4 Reagent kit on the Guava PCA (EMD Millipore, Billerica, MA), the CD4 easy count and the CD4% easy count kit on the CyFlow Counter (Partec, Münster, Germany) and the Pima CD4 cartridge kit on the Alere PIMA analyser (Alere Technologies, Jena, Germany).

Techniques:

Journal: Tropical Medicine & International Health

Article Title: VISITECT ® CD4 advanced disease assay in routine use: Diagnostic accuracy and usability, Ethiopia and Indonesia

doi: 10.1111/tmi.14124

Figure Lengend Snippet: Box‐ and whisker plots showing variations in reference CD4 counts with WHO clinical HIV stage, presence or absence of TB suggestive symptoms, and VISITECT® CD4 Advanced Disease (VISITECT‐CD4‐AD).

Article Snippet: Unfortunately, for commercial reasons, Pima was discontinued in 2022 and FACSPresto in 2023, leaving Omega Diagnostics' (UK) VISITECT® CD4 Advanced Disease assay (VISITECT‐CD4‐AD) as the only option currently for obtaining an estimate of a PLHIV's CD4 count at POC [ ].

Techniques: Whisker Assay

Journal: Tropical Medicine & International Health

Article Title: VISITECT ® CD4 advanced disease assay in routine use: Diagnostic accuracy and usability, Ethiopia and Indonesia

doi: 10.1111/tmi.14124

Figure Lengend Snippet: Specificity of VISITECT‐CD4‐AD with 95% confidence intervals, by study site: (a) Site‐specific specificity over total study period in all 10 health facilities. Size of marker indicates number of participants enrolled. (b) Specificity in Ethiopian health facilities before and after retraining.

Article Snippet: Unfortunately, for commercial reasons, Pima was discontinued in 2022 and FACSPresto in 2023, leaving Omega Diagnostics' (UK) VISITECT® CD4 Advanced Disease assay (VISITECT‐CD4‐AD) as the only option currently for obtaining an estimate of a PLHIV's CD4 count at POC [ ].

Techniques: Marker